-
Yoko SAITO, Makoto KANDATSU
1975 Volume 39 Issue 4 Pages
753-758
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
The amounts of sRNA and rRNA in the muscle of hind limbs and liver were measured in the rats fed on protein free diet for 28 days.
The amounts of sRNA and rRNA in both tissues were decreased exponentially by protein deprivation, and in the muscle a daily fractional loss of sRNA was clearly less than that of rRNA. Thus during the experimental period the amount of sRNA fell unparallel with that of rRNA. This result suggests that synthesis and degradation of both RNAs may be separately controlled by diet.
sRNA content in muscle and liver of rats fed on the 20% casein containing diet were about 27% and 14% of total RNA (sRNA plus rRNA), respectively.
View full abstract
-
Wenlii LIN, Kunio OISHI, Ko AIDA
1975 Volume 39 Issue 4 Pages
759-765
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
A new and practical method for the screening of neuraminidase inhibitors (NI) by means of the viral hemagglutination (HA)-dehemagglutination (deHA) reactions was suggested. The best conditions for the HA and deHA reactions were investigated. Existence of strong inhibition activity on the viral deHA has been recognized in the culture filtrates of some strains of actinomycetes. All of these deHA inhibitors showed NI activity that is not specified to the strain of the test viruses. About 0.25mg/ml of the preparation obtained from the culture filtrate of the strongest actinomycetes, No. 289, inhibited the liberation of neuraminic acid from bovine submaxillary mucin by 80 HA units/ml of influenza A Fukuoka/1/70 (H
3N
2) virus up to 80%.
View full abstract
-
Akira FURUYA, Fumio KATO, Kiyoshi NAKAYAMA
1975 Volume 39 Issue 4 Pages
767-771
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
From the inosine producing mutants of
Brevibacterium ammoniagenes, KY 13761 was selected as a strain which produced 6-azauridine from 6-azauracil.
The conditions for the conversion were examined and the intermitent feeding of 6-azauracil was found effective for the accumulation.
In order to increase the accumulation, prototrophic revertants were induced from KY 13761 and KY 13021 was selected. By intermitent feeding of 6-azauracil of a final concentration of 6mg per ml, a maximal accumulation, 12.4mg/ml, of 6-azauridine was obtained with KY 13021.
View full abstract
-
Shuzo SATOI, Kunio NAKAHARA, Sawao MURAO
1975 Volume 39 Issue 4 Pages
773-778
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Cladosporium sp. No. 45-2, an acid protease-producing microorganism, was cultured in medium containing a microbial acid protease inhibitor (S-PI). By the addition of S-PI, the amount of acid protease in the culture broth showed an increase of 50_??_80%. over those of normal culture (S-PI-free). Acid protease was purified from the S-PI-added culture filtrate, and its enzymatic and physicochemical properties were compared with those of acid protease obtained from normal culture. It was determined that the acid protease obtained from S-PT-added culture was the same as that of normal culture, but that the productivity was increased by the addition of S-PI.
The increase in acid protease productivity is assumed to be due to a change in metabolism by the addition of S-PI.
View full abstract
-
Shojiro IWAHARA, Yasuto KANEMARU
1975 Volume 39 Issue 4 Pages
779-784
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Intact cells of a biotin-producing bacterium, KY-21-1-25, were found to synthesize biotin from dethiobiotin. Optimal conditions for the biosynthesis of biotin from dethio-biotin by intact cells were investigated. Intact cells harvested from adenine-supplemented medium showed intensive biosynthesis. However, the biosynthesis of biotin by intact cells was strongly inhibited by the addition of adenine or adenosine. The inhibitory activity of adenine was about 10-fold greater than that of adenosine. Formation of several unidentified biotin-vitamers was observed in both reaction mixtures incubated with and/or without addition of adenine.
View full abstract
-
Tomohiko MORI, Zen-ichi YOKOYAMA
1975 Volume 39 Issue 4 Pages
785-790
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Ribonucleic acids from the cotyledons of soybean seeds (H-RNA) stimulated the amino acid incorporation in a cell-free system prepared from soybean seedlings at a concentration of 5mM magnesium acetate. In a reticulocyte cell-free system H-RNA stimulated the amino acid incorporation at a concentration of 10mM Mg
2+, but not at 4.3mM Mg
2+ Aurintricarboxylate, an inhibitor for the initiation of protein synthesis, inhibited the H-RNA and TMV-RNA-stimulated amino acid incorporation by about 90% and 70% at a concentration of 50 μM in
E. coli and soybean seedling systems, respectively. When the H-RNA preparation was passed through a Millipore filter, about 1% of the charged RNA was retained on the filter. Chromatography on plain cellulose of H-RNA resulted in slight enrichment of an RNA fraction with the characteristics of mRNA.
View full abstract
-
Ryuhei FUNABIKI, Sigeru IDE, Norio EBE, Osamu TAGUCHI, Kazumi YASUDA
1975 Volume 39 Issue 4 Pages
791-793
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Diurnal changes of glycogen content in skeletal muscle of meal-fed rats under condition of starvation was first clearly demonstrated. The phases of diurnal change of glycogen content in skeletal muscle was the same in liver. Variations of blood glucose level and concentration of serum free fatty acids were discussed in connection with glycogen deposit in liver and skeletal muscle.
View full abstract
-
Hiroki MURAKAMI, Toshihiro MIYOSHI, Michiko KOGA, Koji YAMADA, Hirohis ...
1975 Volume 39 Issue 4 Pages
795-801
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Strand breakages of mammalian cellular chromosomal DNA with aromatic reductones were ascertained by use of a cultured cell strain of the rat fetal lung (RFL). The mode of the breakages was investigated by ultracentrifugal analyses. The reductones induced the breakages of the cellular DNA in two different fashions; one is single strand breaks and another double strand breaks. Although the single strand breaks were rapidly repaired, double strand breaks were only partially repaired. Both breaks were not cytocidal. Some physiological alterations were observed to follow the strand breaks.
View full abstract
-
Mitsuru ANDO, Masayuki NAKAGAWA, Toshiie NAKAMURA, Kazuo TOMITA
1975 Volume 39 Issue 4 Pages
803-809
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Excretion, distribution and metabolism of the insecticide, Isoxathion, administered orally in male Wistar-strain rats, were investigated with a carbon-14 labeled chemical. During 96 hr, approximately 85% and 14% of the total radioactivity were excreted in the urine and feces. Distribution of isoxathion after oral administration in the rats was investigated by means of whole-body autoradiographic technique and measurement of radioactivity in the tissues. At least eleven radioactive metabolites were detected, four of which were structurally determined. They were 3-hydroxy-5-phenylisoxazole, 3-(β-D-glu-copyranuronosyloxy)-5-phenylisoxazole, 5-phenyl-3-isoxazolyl sulfate and hippuric acid.
View full abstract
-
Hiroshi SUZUKI, Bienvenido O. JULIANO
1975 Volume 39 Issue 4 Pages
811-817
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
In the alkaliviscogram of starch of 26 nonwaxy ricer grown in the tropics, gelatinization normality correlated positively with final gelatinization temperature (BEPT) of starch (
r=0.969
**) and negatively with alkali spreading value of milled rice (
r=-0.931
**). Peak viscosity was not linearly related to amylose content. Among samples of rice starch having a high amylose (>28%) content, peak viscosity was correlated with the gel consistency of starch (
r=-0.690
**) and of milled rice (
r=-0.644
**) (
n=18). These high-amylose starches showed the widest variation in peak viscosity. Amylose content, and gel consistency were inherited from the same parent in all nine varieties and lines studied, whereas peak viscosity, gelatinization normality and the final BEPT were inherited from either parent. The starch of five waxy rices showed higher peak viscosities even at a concentration of 1.8% as compared with a 2.0% nonwaxy rice starch.
View full abstract
-
Masayuki SATO, Akira KAJI
1975 Volume 39 Issue 4 Pages
819-824
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
A pectate lyase (EC 4. 2. 2. 2) was purified from the culture filtrate of
Streptomyces fradiae by precipitation with ammonium sulfate, followed by column chromatography on DEAE-Sephadex A-50, SP-Sephadex C-50 and by gel-filtration on Sephadex G-100. The enzyme was an endo-pectate lyase with an optimum activity at pH 9.0_??_9.2, and required only calcium ion for the activity. The enzyme had macerating activity and was active on both native pectin and polygalacturonic acid, but the activity toward oligogalacturonides was small.
View full abstract
-
Shin KUROGOCHI, Satoshi TAHARA, Junya MIZUTANI
1975 Volume 39 Issue 4 Pages
825-831
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
The metabolism of sorbic acid (
trans-2,
trans-4-hexadienoic acid) and its related compounds by
Mucor sp. A-73 was investigated. Sorbic acid was reduced by this fungus to
trans-4-hexenol (more than 90% yield). In a series of hexamonoenoic acids, carboxyl groups and α, β-double bond were reduced, but β, γ and γ, δ double bonds were hardly reduced. The reduction of
cis-2-hexenoic acid was slower than that of the corresponding
trans isomer. Sorbic alcohol, one of α, β-unsaturated alcohols, was converted well to
trans-4-hexenol by the fungus. These results showed that this fungus could carry out two independent reductions: (i) carboxyl group-alcohol, (ii) α, β-unsaturated alcohol→α, β-saturated one. Furthermore, α, β-unsaturated alcohols were temporarily detected in the course of fungal reductions of some α, β-unsaturated acids. The fact suggested that the reduction of α, β-unsaturated acids to α, β-saturated alcohols was initiated by the reaction (i) and followed by (ii). The biological hydrogenation of α, β-unsaturated alcohols is a new reaction.
View full abstract
-
Toshiya KAMIKADO, Ching-Fun CHANG, Shigeo MURAKOSHI, Akira SAKURAI, Sa ...
1975 Volume 39 Issue 4 Pages
833-836
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Two lignans were isolated from leaves of
Magnolia kobus DC. as growth inhibitors on silkworm larvae and structurally elucidated as sesamin (I) and kobusin (II) which has been hitherto unknown.
View full abstract
-
Kinji UCHIDA
1975 Volume 39 Issue 4 Pages
837-842
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Effects of cultural conditions on the fatty acid composition of
Lactobacillus heterohiochii were studied with the special reference to the formation of unusually long chain fatty acids (C
20 and longer ones). The fatty acid composition did not significantly alter throughout cultural ages, and the acids with unusually long chains were synthesized under a variety of conditions. The proportion of the unusual acids rose with an increase in the concentration of yeast-extract in media. No unequivocal relationship between alcohol concentration of media and the chain lengths of the fatty acids was observed. The raise in alcohol concentration resulted in a significant increase of the ratio of total unsaturated to saturated acids (U/S ratio). The U/S ratio decreased with the rise of growth temperature below 25°C, but it stayed constant at temperature higher than 28°C.
View full abstract
-
Yoshihiko HIRAMI, Rikio OHUCHI, Yuichiro KUROSAWA, Hiromu MORI
1975 Volume 39 Issue 4 Pages
843-850
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
1-Methyl-, 4-methyl, 1, 4-dimethyl- and 1-ethyl-4-methyl-1, 3, 5 (10) -estratrien-17 β-amines (V) were synthesized by reduction of the corresponding oximes (IV) with lithium aluminum hydride. 17 α-Amines (X) were obtained by reduction of 17 α-azido compounds (IX) with lithium aluminum hydride. N, N-Bis (2-hydroxyethyl) and 2-cyanoethyl derivatives (VI, XI, VII and XII) were also prepared. Antimicrobial activities of these compounds were tested.
View full abstract
-
Yukihiro ISHIKAWA, Etsuji YUKI
1975 Volume 39 Issue 4 Pages
851-857
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
The structures of many reaction products obtained when various tocopherols (Toc's) and trimethylamine oxide (TMAO) were treated in liquid paraffin under a nitrogen stream at 180°C, were determined and their antioxidative activities were investigated.
The reaction products (Toc dimers) isolated were as follows: α-tocopheryl ethane from α-Toc; 5-(γ-tocopheryloxy)-γ-Toc, 5-(γ-tocopheryl)-γ-Toc (two kinds) and α-tocopheryl ethane from γ-Toc; 5-(δ-tocopheryloxy)-δ-Toc from δ-Toc.
The two 5-(γ-tocopheryl)-γ-Tot's are atropisomers of each other (TLC (
Rf):0.75, 0.45-benzene) and isomerization occurred within 20min when they were treated under nitrogen at 180°C.
All Toc dimers, in particular 5-(γ-tocopheryloxy)-γ-Toc, have antioxidative activities and excellent synergism with TMAO in inhibiting the oxidation of lard kept in the dark at 60°C.
View full abstract
-
Setsuo TAKEUCHI, Yoshiki KONO, Akira KAWARADA, Yasuo OTA, Masayoshi NA ...
1975 Volume 39 Issue 4 Pages
859-861
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Aqueous 70% methanol extract of rice (
Oryza saliva, var. Hadsaduri) hulls was found to contain an effective plant growth regulator, which was located at
Rf 0.7 on a histogram on silica gel (Kieselgel GF254) TLC (iso-PrOH: 14% NH
4OH, 10:2). After preliminary purification by ion exchange, the crude extract was repeatedly purified by column chromatography. The active substance thus obtained as colorless crystals was identical with authentic nicotinamide. The growth promotive effect of nicotinamide in co-operation with ammonium ion on the intact plant at low concentration was observed.
View full abstract
-
Munehiko TANAKA, Tung-Ching LEE, C. O. CHICHESTER
1975 Volume 39 Issue 4 Pages
863-866
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Egg albumin was stored at 37°C, under 68% relative humidity in the presence of excess glucose for 40 days to study the effect of browning on chemical properties of the protein. Binding of glucose to egg albumin occurred mostly in the early stage of browning whereas brown color started developing after 10 days. Amino acid analyses showed large lose in lysine, arginine, serine, histidine and tryptophan. Dye binding methods demonstrated some relationships to the nutritional value of browned egg albumin, however they were not accurate enough to follow the change in the nutritional value of egg albumin during storage. Relatively close correlation between the amount of glucose bound and the loss of nutritional value was observed.
View full abstract
-
Keiichiro NISHIMURA, Tsuneyasu KAWATA, Kozi ASADA, Minoru NAKAJIMA
1975 Volume 39 Issue 4 Pages
867-872
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Some derivatives of phenylurea, N-phenylcarbamate,
s-triazine and acylanilide inhibited the cyclic photophosphorylation of spinach chloroplasts catalyzed by phenazine methosulfate and accelerated the photosystem I-dependent electron flow estimated as the disproportionation of diphenylcarbazone. Acceleration was slightly stimulated by the simultaneous addition of methylamine. Thus, these Hill reaction inhibitors act as uncouplers of cyclic photo-phosphorylation as does methylamine. The inhibiting activities of the chemicals on the photoreduction of ferricyanide and on photophosphorylation had a parabolic relation to the partition coefficient in the octanol-water system of the chemicals.
View full abstract
-
Fumio YAMAUCHI, Misako KAWASE, Meiko KANBE, Kazuo SHIBAZAKI
1975 Volume 39 Issue 4 Pages
873-878
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
Using a Dowex 50-X2 column and eluting with a 1mM sodium acetate buffer (pH 2.6), three L-β-aspartamido-carbohydrate (Asn-carbohydrate) fractions have been isolated in 19% yield from the pronase digest of a 7S soybean protein. The Asn-carbohydrates were composed of one asparagine, two glucosamine and seven, eight and nine mannose residues. Further, 1-L-β-aspartamido-2-acetamido-1, 2-dideoxy-β-D-glucose (Asn-GlcNAc), which is derived from the protein-carbohydrate linkage, was produced by partial hydrolysis of the Asn-carbohydrates.
View full abstract
-
Toshiro SHIDA, Yasuo HOMMA, Akira KAMIMURA, Tomomasa MISATO
1975 Volume 39 Issue 4 Pages
879-883
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
The degradation of N-lauroyl-L-valine in soil and the effects of sunlight and ultraviolet radiation on this compound were studied. When N-lauroyl-L-valine was added to the soil extract, a precipitate was observed. The precipitate was thought to be a salt formed from one calcium atom and two molecules of N-lauroyl-L-valine. These data indicated that N-lauroyl-L-valine was absorbed firmly in the soil. Qualitative analysis of compounds formed during incubation of soil with N-lauroyl-L-valine was investigated by gas chromato-graphy. Laurie acid (C
12) and capric acid (C
10) were detected in the medium after incubation. The amino acid could be determined quantitatively by a microbial assay method. The experiment using N-lauroyl-L-valine (
14C) indicated that
14CO
2 was produced as a final product. These data suggested that N-lauroyl-L-valine was spilt to lauric acid and L-valine, and the lauric acid was degraded to CO
2 through capric acid by β-oxidation. The parent compound, N-lauroyl-L-valine was stable to sunlight and ultraviolet rays.
View full abstract
-
Kanzo SAKATA, Akira SAKURAI, Saburo TAMURA
1975 Volume 39 Issue 4 Pages
885-892
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
On hydrolysis with Dowex 50 W, ezomycin A
1 (1) C
26H
40N
8O
16S gave ezomycin A
2 (2) C
19H
28N
6O
13 and L-cystathionine (3) as main products. Further acid hydrolysis of 2 afforded an amino uronic acid named ezoaminuroic acid (5), 3-amino-3, 4-dideoxy-D-xylohexopyranuroic acid, and cytosine. Ezoaminuroic acid, L-cystathionine and cytosine were found to constitute ezomycin A
1.
View full abstract
-
B. B. MIGICOVSKY, C. MADHOSINGH
1975 Volume 39 Issue 4 Pages
893-898
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
An inhibitor of cholesterol synthesis (ICS) was extracted from the mitochondria of starved rats' liver, and purified by dialysis, tryptic digestion, precipitation at pH 3.0 and Sephadex column chromatography. This preparation was inhibitory to cholesterol synthesis and produced one protein band in acrylamide disc electrophoresis.
The acid precipitate consisted of 68% protein and produced a 20-fold increase in
in vitro inhibition compared to the original ICS. Inhibition by ICS was also demonstrated
in vivo.
View full abstract
-
Naoko HARADA, Tei SATO
1975 Volume 39 Issue 4 Pages
899-900
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
B. N. KOWALE, U. K. MISRA
1975 Volume 39 Issue 4 Pages
901-903
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
Chizuko KITAYAMA, Motoo TSUSUE
1975 Volume 39 Issue 4 Pages
905-906
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
Tomoyoshi IWATSUBO, Hiroki NAKAGAWA, Nagao OGURA, Hidetaro TAKEHANA
1975 Volume 39 Issue 4 Pages
907-908
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
Tetsuya YAMADA, Masanori SAWADA, Motoji TAKI
1975 Volume 39 Issue 4 Pages
909-910
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
Akio KOBAYASHI, Yoshiari SHIBATA, Kyohei YAMASHITA
1975 Volume 39 Issue 4 Pages
911-912
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
Takane FUJIMORI, Reiko KASUGA, Hajime KANEKO, Masao NOGUCHI
1975 Volume 39 Issue 4 Pages
913-914
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
Haruo SETO, Shiro URANO
1975 Volume 39 Issue 4 Pages
915-916
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
-
1975 Volume 39 Issue 4 Pages
e1
Published: 1975
Released on J-STAGE: November 27, 2008
JOURNAL
FREE ACCESS
