Palm oil mill effluent (POME), the by-product at the crude palm oil (CPO) production is released to large Lagoons and treated in acidic ponds, oxidizing ponds and anaerobic ponds. Over the last decades, POME has become a critical environmental issue due to the insufficient conventional treatment process of POME where substantial amounts of greenhouse gases (GHGs) including methane are emitted. Considering the dependency and enormous demand on palm oil globally, urgent action is required to solve this source of methane generation that accounts for 10% of greenhouse gas emissions in Indonesia. The DHA-rich heterotrohic microalga, Aurantichytrium limacinum has been recently employed as sustainable solution to this problem. A. limacinum grew well in fresh POME (also called ByLPO or heavy phase) and produced high amount of DHA. DHA has been reported to have at least 13 kinds of efficacies for human health and therefore sold as health supplement in the market. Our recent findings were summarized as follows; 1) ethanol extract of Aurantiochytrium shows anti-stress effect, 2) fisheries and hen’s feeds containing dry material of Auratiochitrium increased DHA content in Kuruma prawn, sea bream, and egg of hens. This manuscript will introduce the current status of process verification by using a demo plant at 10 metric-ton-scale which has been installed in North-Sumatra, Indonesia.
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